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PUS3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PUS3 Antibody (N-term) AP10835a
    PUS3 Antibody (N-term) (Cat. #AP10835a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUS3 antibody detected the PUS3 protein (arrow).
  • 14 - PUS3 Antibody (N-term) AP10835a
    PUS3 antibody (N-term) (Cat. #AP10835a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUS3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9BZE2
Other Accession NP_112597.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 55647 Da
Antigen Region 35-64 aa
Additional Information
Gene ID 83480
Other Names tRNA pseudouridine(38/39) synthase, tRNA pseudouridine synthase 3, tRNA pseudouridylate synthase 3, tRNA-uridine isomerase 3, PUS3
Target/Specificity This PUS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-64 amino acids from the N-terminal region of human PUS3.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPUS3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PUS3
Function Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs.
Cellular Location Nucleus.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs (By similarity).

REFERENCES

Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Chen, J., et al. Biochemistry 39(41):12723-12730(2000)

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