IGHG1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-IGHG1 Antibody (Center) at 1:2000 dilution Lane 1: human fetal spleen lysate Lane 2: human fetal thymus lysate Lane 3: human spleen lysate Lane 4: human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
IGHG1 Antibody (Center) (Cat. #AP10959c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	P01857
Other Accession	P01860
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	154-180 aa

Additional Information
Other Names	Ig gamma-1 chain C region, IGHG1
Target/Specificity	This IGHG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-180 amino acids from the Central region of human IGHG1.
Dilution	WB~~1:2000 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	IGHG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.