癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NUDT15 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| IHC-P, FC, WB, E |
|---|---|
| Primary Accession | Q9NV35 |
| Other Accession | NP_060753.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 18609 Da |
| Antigen Region | 89-118 aa |
| Gene ID | 55270 |
|---|---|
| Other Names | Probable 8-oxo-dGTP diphosphatase NUDT15, 8-oxo-dGTPase NUDT15, 8-dihydro-8-oxoguanine-triphosphatase NUDT15, MutT homolog 2, MTH2, Nucleoside diphosphate-linked moiety X motif 15, Nudix motif 15, NUDT15, MTH2 |
| Target/Specificity | This NUDT15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the C-terminal region of human NUDT15. |
| Dilution | IHC-P~~1:100~500 FC~~1:10~50 WB~~1:500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NUDT15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NUDT15 (HGNC:23063) |
|---|---|
| Function | May catalyze the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms like 8-oxo-dGTP and the prodrug thiopurine derivatives 6-thio-dGTP and 6-thio-GTP (PubMed:26238318). Could also catalyze the hydrolysis of some nucleoside diphosphate derivatives (PubMed:22556419, PubMed:26238318). Hydrolyzes oxidized nucleosides triphosphates like 8-oxo-dGTP in vitro, but the specificity and efficiency towards these substrates are low. Therefore, the potential in vivo sanitizing role of this enzyme, that would consist in removing oxidatively damaged forms of nucleosides to prevent their incorporation into DNA, is unclear (PubMed:22556419, PubMed:26238318). Through the hydrolysis of thioguanosine triphosphates may participate in the catabolism of thiopurine drugs (PubMed:25108385, PubMed:26238318). May also have a role in DNA synthesis and cell cycle progression by stabilizing PCNA (PubMed:19419956). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear (By similarity). May have a role in DNA synthesis and cell cycle progression throught the interaction with PCNA.
REFERENCES
Hori, M., et al. Free Radic. Biol. Med. 48(9):1197-1201(2010) Yu, Y., et al. J. Biol. Chem. 284(29):19310-19320(2009) Dunham, A., et al. Nature 428(6982):522-528(2004) Cai, J.P., et al. Biochem. Biophys. Res. Commun. 305(4):1073-1077(2003)
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