C10orf72 Antibody (Center) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes: Anti-C10orf72 Antibody (Center) at 1:1000 dilution + RPMI 8226 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 36 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
C10orf72 Antibody (Center) (Cat. #AP11192c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the C10orf72 antibody detected the C10orf72 protein (arrow).
C10orf72 Antibody (Center) (Cat. #AP11192c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q8IW00
Other Accession	NP_659421.1, NP_001026916.2
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	36146 Da
Antigen Region	142-170 aa

Additional Information
Gene ID	196740
Other Names	V-set and transmembrane domain-containing protein 4, Peptide Lv, VSTM4, C10orf72
Target/Specificity	This C10orf72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-170 amino acids from the Central region of human C10orf72.
Dilution	WB~~1:1000 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	C10orf72 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	VSTM4
Synonyms	C10orf72
Function	Peptide Lv enhances L-type voltage-gated calcium channel (L- VGCC) currents in retinal photoreceptors.
Cellular Location	[Peptide Lv]: Secreted {ECO:0000250\|UniProtKB:T1NXB5}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.