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GGH Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - GGH Antibody (N-term) AP11236a
    Anti-GGH Antibody (N-term) at 1:2000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - GGH Antibody (N-term) AP11236a
    GGH Antibody (N-term) (Cat. #AP11236a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GGH Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - GGH Antibody (N-term) AP11236a
    GGH Antibody (N-term) (Cat. #AP11236a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession Q92820
Other Accession NP_003869
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 7-34 aa
Additional Information
Other Names Gamma-glutamyl hydrolase, Conjugase, GH, Gamma-Glu-X carboxypeptidase, GGH
Target/Specificity This GGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human GGH.
Dilution IHC-P~~1:100~500
FC~~1:10~50
WB~~1:2000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGGH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq].

REFERENCES

Adjei, A.A., et al. J Thorac Oncol 5(9):1346-1353(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Organista-Nava, J., et al. Leuk. Res. 34(6):728-732(2010)
Figueiredo, J.C., et al. Cancer Causes Control 21(4):597-608(2010)
Dervieux, T., et al. Pharmacogenet. Genomics (2009) In press :

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