C4orf39 Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-C4orf39 Antibody (C-term) at 1:500 dilution Lane 1: SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 19kDa Blocking/Dilution buffer: 5% NFDM/TBST.
C4orf39 Antibody (C-term) (Cat. #AP11347b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the C4orf39 antibody detected the C4orf39 protein (arrow).
C4orf39 Antibody (C-term) (Cat. #AP11347b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of C4orf39 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of C4orf39 Antibody (C-term)(Cat#AP11347b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
C4orf39 Antibody (C-term) (Cat. #AP11347b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, FC, E
Primary Accession	Q96MZ4
Other Accession	NP_694572.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	17021 Da
Antigen Region	98-127 aa

Additional Information
Other Names	Protein FAM218A, FAM218A, C4orf39
Target/Specificity	This C4orf39 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-127 amino acids from the C-terminal region of human C4orf39.
Dilution	WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	C4orf39 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.