WDR70 Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-WDR70 Antibody (C-term) at 1:500 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
WDR70 Antibody (C-term) (Cat. #AP11355b)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WDR70 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
WDR70 Antibody (C-term) (Cat. #AP11355b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of WDR70 Antibody (C-term)(Cat#AP11355b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, IF, E
Primary Accession	Q9NW82
Other Accession	Q5EB92, Q3TWF6, Q32LB0, NP_060504.1
Reactivity	Human, Rat, Mouse
Predicted	Mouse, Rat, Bovine, Canine, Rabbit, Chicken
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	73201 Da
Antigen Region	575-604 aa

Additional Information
Gene ID	55100
Other Names	WD repeat-containing protein 70, WDR70
Target/Specificity	This WDR70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-604 amino acids from the C-terminal region of human WDR70.
Dilution	WB~~1:500 IHC-P~~1:100~500 FC~~1:10~50 IF~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	WDR70 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.