TMEM150B Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-TMEM150B Antibody (N-term) at 1:1000 dilution Lane 1 : Caco2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 36kDa Blocking/Dilution buffer : 5% NFDM/TBST.
TMEM150B Antibody (N-term) (Cat. #AP11399a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TMEM150B antibody detected the TMEM150B protein (arrow).
TMEM150B Antibody (N-term) (Cat. #AP11399a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TMEM150B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of TMEM150B Antibody (N-term)(Cat#AP11399a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, E
Primary Accession	A6NC51
Other Accession	NP_001078957.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	25701 Da
Antigen Region	13-41 aa

Additional Information
Gene ID	284417
Other Names	Transmembrane protein 150B, Transmembrane protein 224, TMEM150B, TMEM224
Target/Specificity	This TMEM150B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-41 amino acids from the N-terminal region of human TMEM150B.
Dilution	WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TMEM150B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TMEM150B (HGNC:34415)
Function	Modulator of macroautophagy that causes accumulation of autophagosomes under basal conditions and enhances autophagic flux (PubMed:25929859). Represses cell death and promotes long-term clonogenic survival of cells grown in the absence of glucose in a macroautophagy-independent manner (PubMed:25929859). May have some role in extracellular matrix engulfment or growth factor receptor recycling, both of which can modulate cell survival (PubMed:25929859).
Cellular Location	Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Note=Localizes mainly at the plasma membrane where it concentrates at actin-rich focal adhesions (PubMed:25608530, PubMed:25929859).
Tissue Location	Highly expressed in the colon and lung with comparatively high levels also detectable in the lymph nodes, placenta, duodenum, peripheral blood mononuclear cells and spleen (PubMed:25929859).