C6orf138 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

C6orf138 Antibody (Center) (Cat. #AP11405c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the C6orf138 antibody detected the C6orf138 protein (arrow).
C6orf138 Antibody (Center) (Cat. #AP11405c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of C6orf138 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q6ZW05
Other Accession	B9EKX1, NP_997382.2, NP_001013754.3
Reactivity	Human
Predicted	Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	96371 Da
Antigen Region	563-591 aa

Additional Information
Gene ID	442213
Other Names	Patched domain-containing protein 4, PTCHD4, C6orf138
Target/Specificity	This C6orf138 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 563-591 amino acids from the Central region of human C6orf138.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	C6orf138 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	PTCHD4
Synonyms	C6orf138, PTCH53
Function	Could act as a repressor of canonical hedgehog signaling by antagonizing the effects of SMO, as suggested by down-regulation of hedgehog target genes, including GLI1, PTCH1, and PTCH2 in PTCHD4- expressing cells.
Cellular Location	Membrane; Multi-pass membrane protein

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.