LMTK3 Antibody (N-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Overlay histogram showing U-2 OS cells stained with AP11540a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11540a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-LMTK3 Antibody (N-term) at 1:1000-1;2000 dilution Lane 1: Hela lysate Lane 2: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 154 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q96Q04
Other Accession	Q5XJV6, XP_055866.6
Reactivity	Human, Mouse
Predicted	Rat, Bovine, Canine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	153661 Da
Antigen Region	156-187 aa

Additional Information
Gene ID	114783
Other Names	Serine/threonine-protein kinase LMTK3, Lemur tyrosine kinase 3, LMTK3, KIAA1883, TYKLM3
Target/Specificity	This LMTK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-187 amino acids from the N-terminal region of human LMTK3.
Dilution	WB~~1:1000-1;2000 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	LMTK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	LMTK3
Synonyms	KIAA1883, TYKLM3
Function	Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (PubMed:21602804). Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons (By similarity).
Cellular Location	Membrane {ECO:0000250\|UniProtKB:Q5XJV6}; Single- pass membrane protein {ECO:0000250\|UniProtKB:Q5XJV6}. Cell projection, axon {ECO:0000250\|UniProtKB:Q5XJV6}. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q5XJV6}. Golgi apparatus membrane {ECO:0000250\|UniProtKB:Q5XJV6}. Note=Punctate pattern in cell projections

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.