癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
RASGRP2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q7LDG7 |
| Other Accession | P0C643, Q9QUG9, A6N9I4, NP_001092141.1, NP_722541.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 69248 Da |
| Antigen Region | 7-34 aa |
| Gene ID | 10235 |
|---|---|
| Other Names | RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, Cdc25-like protein, hCDC25L, F25B33 kinase-like protein, RASGRP2, CDC25L, MCG7 |
| Target/Specificity | This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human RASGRP2. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RASGRP2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RASGRP2 |
|---|---|
| Synonyms | CDC25L, MCG7 |
| Function | Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. |
| Cellular Location | Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Synapse, synaptosome. Cell projection, ruffle membrane; Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F- actin. Localizes to the cell bodies and axons of striatal neurons (By similarity). |
| Tissue Location | Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.
REFERENCES
Nagamine, K., et al. Biol. Pharm. Bull. 33(7):1138-1142(2010)
Katagiri, K., et al. J. Biol. Chem. 279(12):11875-11881(2004)
Eto, K., et al. Proc. Natl. Acad. Sci. U.S.A. 99(20):12819-12824(2002)
Toki, S., et al. J. Comp. Neurol. 437(4):398-407(2001)
Guo, F.F., et al. J. Biol. Chem. 276(27):25568-25581(2001)
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