癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NEU2 Antibody (N-term) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P-Leica, IF, FC, E |
|---|---|
| Primary Accession | Q9Y3R4 |
| Other Accession | NP_005374.2 |
| Reactivity | Human |
| Predicted | Canine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 42254 Da |
| Antigen Region | 23-50 aa |
| Gene ID | 4759 |
|---|---|
| Other Names | Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2, NEU2 |
| Target/Specificity | This NEU2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human NEU2. |
| Dilution | WB~~1:1000 IHC-P-Leica~~N/A IF~~1:10~50 FC~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NEU2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | NEU2 (HGNC:7759) |
|---|---|
| Function | Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides (PubMed:14613940, PubMed:22228546). Recognizes sialyl linkage positions of the glycan moiety as well as the supramolecular organization of the sialoglycoconjugate. Displays preference for alpha- (2->3)-sialylated GD1a and GT1B gangliosides over alpha-(2->8)- sialylated GD1b, in both monomeric forms and micelles. Hydrolyzes monomeric GM1 ganglioside, but has no activity toward the miscellar form (PubMed:14613940). Has lower sialidase activity for glycoproteins such as fetuin and TF/transferrin that carry a mixture of alpha-(2->3) and alpha-(2->6)-sialyl linkages. Cleaves milk oligosaccharide alpha- (2->3)-sialyllactose, but is inactive toward alpha-(2->6)-sialyllactose isomer. Has no activity toward colominic acid, a homomer of alpha- (2->8)-linked Neu5Ac residues (PubMed:14613940). |
| Cellular Location | Cytoplasm, cytosol. |
| Tissue Location | Expressed in skeletal muscle, fetal liver and embryonic carcinoma cell line NT2-D1. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq].
REFERENCES
Stoppani, E., et al. Cell Biol. Int. 33(9):1020-1025(2009)
Li, C.Y., et al. Cell Res. 17(4):357-362(2007)
Chavas, L.M., et al. J. Biol. Chem. 280(1):469-475(2005)
Seyrantepe, V., et al. J. Biol. Chem. 279(35):37021-37029(2004)
Tringali, C., et al. J. Biol. Chem. 279(5):3169-3179(2004)
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