癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
MED14 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, FC, E |
|---|---|
| Primary Accession | O60244 |
| Other Accession | NP_004220 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 160607 Da |
| Antigen Region | 586-615 aa |
| Gene ID | 9282 |
|---|---|
| Other Names | Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, ARC150, Cofactor required for Sp1 transcriptional activation subunit 2, CRSP complex subunit 2, Mediator complex subunit 14, RGR1 homolog, hRGR1, Thyroid hormone receptor-associated protein complex 170 kDa component, Trap170, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component, DRIP150, MED14 |
| Target/Specificity | This MED14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from the Central region of human MED14. |
| Dilution | WB~~1:2000 FC~~1:25 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MED14 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MED14 |
|---|---|
| Synonyms | ARC150, CRSP2, CXorf4, DRIP150, EXLM1, R |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Cellular Location | Nucleus. |
| Tissue Location | Ubiquitous. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.
REFERENCES
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Lee, J., et al. Arch. Biochem. Biophys. 461(2):200-210(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Chen, W., et al. Mol. Endocrinol. 20(3):560-572(2006)
终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问，联系：0512-88856768 tech-china@abcepta.com.
