PRAMEF8 Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

PRAMEF8 Antibody (C-term) (Cat. #AP12211b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRAMEF8 antibody detected the PRAMEF8 protein (arrow).
PRAMEF8 Antibody (C-term) (Cat. #AP12211b)immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRAMEF8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
PRAMEF8 Antibody (C-term) (Cat. #AP12211b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q5VWM4
Other Accession	A6NMC2, O95522, NP_001012276.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	53655 Da
Antigen Region	419-447 aa

Additional Information
Gene ID	391002
Other Names	PRAME family member 8, PRAMEF8
Target/Specificity	This PRAMEF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-447 amino acids from the C-terminal region of human PRAMEF8.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PRAMEF8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.