FOXO6 Antibody (Center) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes: Anti-FOXO6 Antibody (Center) at 1:1000 dilution Lane 1: U-20S whole cell lysate Lane 2: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 50. 5 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent confocal image of U251 cell stained with FOXO6 Antibody (Center)(Cat#AP12340c).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXO6 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXO6 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, E
Primary Accession	A8MYZ6
Other Accession	Q70KY4, XP_002342143.1
Reactivity	Mouse
Predicted	Rat, Bovine, Canine, Rabbit, Chicken
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	50595 Da
Antigen Region	154-181 aa

Additional Information
Other Names	Forkhead box protein O6, FOXO6
Target/Specificity	This FOXO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-181 amino acids from the Central region of human FOXO6.
Dilution	WB~~1:1000 IF~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	FOXO6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FOXO6
Function	Transcriptional activator.
Cellular Location	Cytoplasm. Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00089}. Note=When phosphorylated, translocated from nucleus to cytoplasm. High nuclear localization after stimulation with growth factors (By similarity).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.