癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PITRM1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, E |
|---|---|
| Primary Accession | Q5JRX3 |
| Other Accession | NP_055704.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 117413 Da |
| Antigen Region | 490-518 aa |
| Gene ID | 10531 |
|---|---|
| Other Names | Presequence protease, mitochondrial, hPreP, 3424-, Pitrilysin metalloproteinase 1, Metalloprotease 1, hMP1, PITRM1, KIAA1104, MP1 |
| Target/Specificity | This PITRM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 490-518 amino acids from the Central region of human PITRM1. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PITRM1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PITRM1 (HGNC:17663) |
|---|---|
| Function | Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing (PubMed:10360838, PubMed:16849325, PubMed:19196155, PubMed:24931469). Has an ATP-independent activity (PubMed:16849325). Specifically cleaves peptides in the range of 5 to 65 residues (PubMed:19196155). Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference (PubMed:10360838, PubMed:19196155, PubMed:24931469). Degrades the transit peptides of mitochondrial proteins after their cleavage (PubMed:19196155). Also degrades other unstructured peptides (PubMed:19196155). It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion (PubMed:16849325, PubMed:24931469, PubMed:26697887). It is a highly efficient protease, at least toward amyloid-beta protein 40 (PubMed:24931469, PubMed:29383861, PubMed:29764912). Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently (PubMed:24931469). It is also able to degrade amyloid-beta protein 42 (PubMed:29764912). |
| Cellular Location | Mitochondrion. Mitochondrion matrix |
| Tissue Location | Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.
REFERENCES
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Pinho, C.M., et al. Neurosci. Lett. 469(2):204-208(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Yoshida, T., et al. Int. J. Mol. Med. 24(4):539-547(2009)
Chow, K.M., et al. Biochemistry 48(13):2868-2877(2009)
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