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LGALS4 Antibody (N-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - LGALS4 Antibody (N-term) AP12391a
    Anti-LGALS4 Antibody (N-term) at 1:1000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - LGALS4 Antibody (N-term) AP12391a
    LGALS4 Antibody (N-term) (Cat. #AP12391a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the LGALS4 antibody detected the LGALS4 protein (arrow).
  • 4 - LGALS4 Antibody (N-term) AP12391a
    LGALS4 Antibody (N-term) (Cat. #AP12391a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P56470
Other Accession NP_006140.1
Reactivity Human
Predicted Canine, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35941 Da
Antigen Region 70-98 aa
Additional Information
Gene ID 3960
Other Names Galectin-4, Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4, LGALS4
Target/Specificity This LGALS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human LGALS4.
Dilution WB~~1:1000
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLGALS4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name LGALS4
Function Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
Research Areas

BACKGROUND

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer.

REFERENCES

Zimbardi, A.L., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 5), 542-545 (2010) :
Stechly, L., et al. Traffic 10(4):438-450(2009)
Tripodi, D., et al. BMC Med Genomics 2, 65 (2009) :
Oberg, C.T., et al. Bioorg. Med. Chem. Lett. 18(13):3691-3694(2008)
Ideo, H., et al. J. Biol. Chem. 282(29):21081-21089(2007)

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