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SNRPD3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Immunohistochemical analysis of paraffin-embedded Human colon tissue using AP12451A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP12451A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Anti-SNRPD3 Antibody (N-term) at 1:2000 dilution + COLO 205 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-SNRPD3 Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: HL-60 whole cell lysate Lane 6: Mouse heart tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情
文献引用 : 1
实验流程
背景知识

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P-Leica, WB, E
Primary Accession	P62318
Other Accession	P62323, P62320, O44437, NP_004166.1
Reactivity	Human, Mouse
Predicted	Xenopus, Drosophila
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Gene ID	6634
Other Names	Small nuclear ribonucleoprotein Sm D3, Sm-D3, snRNP core protein D3, SNRPD3
Target/Specificity	This SNRPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human SNRPD3.
Dilution	IHC-P-Leica~~1:1000 WB~~1:4000
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SNRPD3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SNRPD3
Function	Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:25555158, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346). Component of both the pre- catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:28502770, PubMed:28781166, PubMed:28076346). Is also a component of the minor U12 spliceosome (PubMed:15146077). As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing (By similarity).
Cellular Location	Cytoplasm, cytosol. Nucleus Note=SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.

Research Areas

A selective inhibitor of PRMT5 with in vivo and in vitro potency in MCL models.
Author : Chan-Penebre E¹, Kuplast KG¹, Majer CR², Boriack-Sjodin PA¹, Wigle TJ², Johnston LD¹, Rioux N¹, Munchhof MJ¹, Jin L³, Jacques SL¹, West KA^{1< Nat Chem Biol. 2015 Apr 27. doi: 10.1038/nchembio.1810. [Epub ahead of print]}	25915199

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The protein encoded by this gene belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.

REFERENCES

Gonsalvez, G.B., et al. J. Cell Biol. 178(5):733-740(2007)
Ma, Y., et al. Structure 13(6):883-892(2005)
Mahler, M., et al. Arthritis Res. Ther. 7 (1), R19-R29 (2005) :
Ong, S.E., et al. Nat. Methods 1(2):119-126(2004)
Ong, S.E., et al. Nat. Methods 1(2):119-126(2004)

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¥ 1,100.00

Cat# AP12451A

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Price:

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SNRPD3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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