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SNRPD3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 59 - SNRPD3 Antibody (N-term) AP12451A
    Immunohistochemical analysis of paraffin-embedded Human colon tissue using AP12451A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - SNRPD3 Antibody (N-term) AP12451A
    Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP12451A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - SNRPD3 Antibody (N-term) AP12451A
    Anti-SNRPD3 Antibody (N-term) at 1:2000 dilution + COLO 205 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SNRPD3 Antibody (N-term) AP12451A
    All lanes : Anti-SNRPD3 Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: HL-60 whole cell lysate Lane 6: Mouse heart tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession P62318
Other Accession P62323, P62320, O44437, NP_004166.1
Reactivity Human, Mouse
Predicted Xenopus, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 13916 Da
Antigen Region 9-38 aa
Additional Information
Gene ID 6634
Other Names Small nuclear ribonucleoprotein Sm D3, Sm-D3, snRNP core protein D3, SNRPD3
Target/Specificity This SNRPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human SNRPD3.
Dilution IHC-P-Leica~~1:500
WB~~1:4000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSNRPD3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNRPD3
Function Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:25555158, PubMed:26912367, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:28076346, PubMed:28502770, PubMed:28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932). As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing (By similarity).
Cellular Location Cytoplasm, cytosol. Nucleus. Note=SMN- mediated assembly into core snRNPs occurs in the cytosol before SMN- mediated transport to the nucleus to be included in spliceosomes
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The protein encoded by this gene belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.

REFERENCES

Gonsalvez, G.B., et al. J. Cell Biol. 178(5):733-740(2007)
Ma, Y., et al. Structure 13(6):883-892(2005)
Mahler, M., et al. Arthritis Res. Ther. 7 (1), R19-R29 (2005) :
Ong, S.E., et al. Nat. Methods 1(2):119-126(2004)
Ong, S.E., et al. Nat. Methods 1(2):119-126(2004)

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