|Application ||WB, E|
|Other Accession||NP_001035259.1, NP_000861.1, NP_955525.1, NP_001035262.2|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||43761 Da|
|Other Names||5-hydroxytryptamine receptor 4, 5-HT-4, 5-HT4, Serotonin receptor 4, HTR4|
|Target/Specificity||This HTR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HTR4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HTR4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Endosome. Note=Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.|
|Tissue Location||Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle|
Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described.
Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010) Maillet, M., et al. Biochem. J. 387 (PT 2), 463-471 (2005) : Brattelid, T., et al. Naunyn Schmiedebergs Arch. Pharmacol. 369(6):616-628(2004) Hiroi, T., et al. Biochem. Biophys. Res. Commun. 289(2):337-344(2001) Bender, E., et al. J. Neurochem. 74(2):478-489(2000)