癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
POFUT2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9Y2G5 |
| Other Accession | NP_598368.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49976 Da |
| Antigen Region | 228-257 aa |
| Gene ID | 23275 |
|---|---|
| Other Names | GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2, O-FucT-2, POFUT2, C21orf80, FUT13, KIAA0958 |
| Target/Specificity | This POFUT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the Central region of human POFUT2. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POFUT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POFUT2 |
|---|---|
| Synonyms | C21orf80, FUT13, KIAA0958 |
| Function | Catalyzes the reaction that attaches fucose through an O- glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X-X-S/T-C2 of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively (PubMed:22588082). O-fucosylates members of several protein families including the ADAMTS, the thrombospondin (TSP) and spondin families (Probable) (PubMed:17395588). Required for the proper secretion of ADAMTS family members such as ADAMTSL1 and ADAMTS13 (PubMed:17395588, PubMed:17395589). The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). |
| Cellular Location | Endoplasmic reticulum. Golgi apparatus. Note=Mainly located in the endoplasmic reticulum. |
| Tissue Location | Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).
REFERENCES
Feng, T., et al. Hum. Genet. 128(3):269-280(2010)
Luo, Y., et al. J. Biol. Chem. 281(14):9385-9392(2006)
Luo, Y., et al. J. Biol. Chem. 281(14):9393-9399(2006)
Menzel, O., et al. Genomics 84(2):320-330(2004)
Martinez-Duncker, I., et al. Glycobiology 13 (12), 1C-5C (2003) :
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