GUF1 Antibody (N-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-GUF1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
GUF1 Antibody (N-term) (Cat. #AP12858a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GUF1 antibody detected the GUF1 protein (arrow).
GUF1 Antibody (N-term) (Cat. #AP12858a)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GUF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q8N442
Other Accession	NP_068746.2
Reactivity	Human
Predicted	Mouse, Rat, Bovine, Canine, Rabbit, Chicken
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	74328 Da
Antigen Region	183-212 aa

Additional Information
Gene ID	60558
Other Names	Translation factor GUF1, mitochondrial {ECO:0000255\|HAMAP-Rule:MF_03137}, 365-, Elongation factor 4 homolog {ECO:0000255\|HAMAP-Rule:MF_03137}, EF-4 {ECO:0000255\|HAMAP-Rule:MF_03137}, GTPase GUF1 {ECO:0000255\|HAMAP-Rule:MF_03137}, Ribosomal back-translocase {ECO:0000255\|HAMAP-Rule:MF_03137}, GUF1 {ECO:0000255\|HAMAP-Rule:MF_03137}
Target/Specificity	This GUF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the N-terminal region of human GUF1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GUF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GUF1 {ECO:0000255\|HAMAP-Rule:MF_03137}
Function	Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.
Cellular Location	Mitochondrion inner membrane {ECO:0000255\|HAMAP- Rule:MF_03137}; Peripheral membrane protein {ECO:0000255\|HAMAP- Rule:MF_03137}; Matrix side {ECO:0000255\|HAMAP-Rule:MF_03137}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.