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SIM1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Immunohistochemical analysis of AP12960a on paraffin-embedded Human kidney tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
Immunohistochemical analysis of AP12960a on paraffin-embedded Human brain tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
Anti-SIM1 Antibody (N-term) at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysate from 293T cell line, using SIM1 Antibody (N-term) (Cat. #AP12960a). AP12960a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

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Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P-Leica, WB, E
Primary Accession	P81133
Other Accession	P05709, Q61045, NP_005059.2
Reactivity	Human
Predicted	Mouse, Drosophila
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Other Names	Single-minded homolog 1, Class E basic helix-loop-helix protein 14, bHLHe14, SIM1, BHLHE14
Target/Specificity	This SIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIM1.
Dilution	IHC-P-Leica~~1:250 WB~~1:1000
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SIM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SIM1
Synonyms	BHLHE14
Function	Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.
Cellular Location	Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00632, ECO:0000255\|PROSITE-ProRule:PRU00981} EMBL; U70212; AAB62395.1; -; mRNA EMBL; AL121948; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; Z86062; -; NOT_ANNOTATED_CDS; Genomic_DNA CCDS; CCDS5045.1; - RefSeq; NP_005059.2; NM_005068.2 RefSeq; XP_005267157.1; XM_005267100.2 RefSeq; XP_016866686.1; XM_017011197.1 UniGene; Hs.520293; - UniGene; Hs.735754; - ProteinModelPortal; P81133; - SMR; P81133; - BioGrid; 112383; 4 IntAct; P81133; 1 STRING; 9606.ENSP00000262901; - iPTMnet; P81133; - PhosphoSitePlus; P81133; - BioMuta; SIM1; - DMDM; 109940166; - PaxDb; P81133; - PeptideAtlas; P81133; - PRIDE; P81133; - ProteomicsDB; 57691; - DNASU; 6492; - Ensembl; ENST00000262901; ENSP00000262901; ENSG00000112246 Ensembl; ENST00000369208; ENSP00000358210; ENSG00000112246 GeneID; 6492; - KEGG; hsa:6492; - UCSC; uc063qgu.1; human CTD; 6492; - DisGeNET; 6492; - EuPathDB; HostDB:ENSG00000112246.9; - GeneCards; SIM1; - HGNC; HGNC:10882; SIM1 HPA; HPA024385; - MalaCards; SIM1; - MIM; 603128; gene neXtProt; NX_P81133; - OpenTargets; ENSG00000112246; - Orphanet; 171829; 6q16 deletion syndrome Orphanet; 369873; Obesity due to SIM1 deficiency Orphanet; 398079; Prader-Willi-like syndrome due to a point mutation PharmGKB; PA35782; - eggNOG; KOG3559; Eukaryota eggNOG; ENOG410XY57; LUCA GeneTree; ENSGT00940000156143; - HOGENOM; HOG000236298; - HOVERGEN; HBG003936; - InParanoid; P81133; - KO; K09100; - OMA; ERPGSQH; - OrthoDB; 231698at2759; - PhylomeDB; P81133; - TreeFam; TF317772; - SIGNOR; P81133; - ChiTaRS; SIM1; human GeneWiki; SIM1; - GenomeRNAi; 6492; - PRO; PR:P81133; - Proteomes; UP000005640; Chromosome 6 Bgee; ENSG00000112246; Expressed in 66 organ(s), highest expression level in quadriceps femoris Genevisible; P81133; HS GO; GO:0005634; C:nucleus; IBA:GO_Central GO; GO:0003677; F:DNA binding; TAS:ProtInc GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:ProtInc GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW GO; GO:0007399; P:nervous system development; TAS:ProtInc GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central GO; GO:0001657; P:ureteric bud development; IEA:Ensembl CDD; cd00083; HLH; 1 CDD; cd00130; PAS; 2 Gene3D; 4.10.280.10; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf InterPro; IPR001610; PAC InterPro; IPR000014; PAS InterPro; IPR035965; PAS-like_dom_sf InterPro; IPR013767; PAS_fold InterPro; IPR013655; PAS_fold_3 InterPro; IPR010578; SIM_C Pfam; PF00010; HLH; 1 Pfam; PF00989; PAS; 1 Pfam; PF08447; PAS_3; 1 Pfam; PF06621; SIM_C; 1 SMART; SM00353; HLH; 1 SMART; SM00086; PAC; 1 SMART; SM00091; PAS; 2 SUPFAM; SSF47459; SSF47459; 1 SUPFAM; SSF55785; SSF55785; 2 PROSITE; PS50888; BHLH; 1 PROSITE; PS50112; PAS; 2 PROSITE; PS51302; SIM_C; 1 2: Evidence at transcript level; Complete proteome; Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation CHAIN 1 766 Single-minded homolog 1 /FTId=PRO_0000127439 DOMAIN 1 53 bHLH. {ECO:0000255\|PROSITE- ProRule:PRU00981} DOMAIN 77 147 PAS 1. {ECO:0000255\|PROSITE- ProRule:PRU00140} DOMAIN 218 288 PAS 2. {ECO:0000255\|PROSITE- ProRule:PRU00140} DOMAIN 292 335 PAC DOMAIN 336 766 Single-minded C-terminal {ECO:0000255\|PROSITE-ProRule:PRU00632} MOTIF 368 387 Nuclear localization signal VARIANT 175 175 L -> F (in dbSNP:rs438766) /FTId=VAR_049549 VARIANT 352 352 P -> T (in dbSNP:rs3734354) /FTId=VAR_034496 VARIANT 371 371 A -> V (in dbSNP:rs3734355) /FTId=VAR_034497 CONFLICT 30 30 P -> A (in Ref. 1; AAB62395) CONFLICT 37 37 L -> V (in Ref. 1; AAB62395) SEQUENCE 766 AA; 85515 MW; 05988D428A84431F CRC64; MKEKSKNAAR TRREKENSEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL KMRVVFPEGL GEAWGHSSRT SPLDNVGREL GSHLLQTLDG FIFVVAPDGK IMYISETASV HLGLSQVELT GNSIYEYIHP ADHDEMTAVL TAHQPYHSHF VQEYEIERSF FLRMKCVLAK RNAGLTCGGY KVIHCSGYLK IRQYSLDMSP FDGCYQNVGL VAVGHSLPPS AVTEIKLHSN MFMFRASLDM KLIFLDSRVA ELTGYEPQDL IEKTLYHHVH GCDTFHLRCA HHLLLVKGQV TTKYYRFLAK HGGWVWVQSY ATIVHNSRSS RPHCIVSVNY VLTDTEYKGL QLSLDQISAS KPAFSYTSSS TPTMTDNRKG AKSRLSSSKS KSRTSPYPQY SGFHTERSES DHDSQWGGSP LTDTASPQLL DPADRPGSQH DASCAYRQFS DRSSLCYGFA LDHSRLVEER HFHTQACEGG RCEAGRYFLG TPQAGREPWW GSRAALPLTK ASPESREAYE NSMPHIASVH RIHGRGHWDE DSVVSSPDPG SASESGDRYR TEQYQSSPHE PSKIETLIRA TQQMIKEEEN RLQLRKAPSD QLASINGAGK KHSLCFANYQ QPPPTGEVCH GSALANTSPC DHIQQREGKM LSPHENDYDN SPTALSRISS PNSDRISKSS LILAKDYLHS DISPHQTAGD HPTVSPNCFG SHRQYFDKHA YTLTGYALEH LYDSETIRNY SLGCNGSHFD VTSHLRMQPD PAQGHKGTSV IITNGS

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome.

REFERENCES

Ghoussaini, M., et al. Obesity (Silver Spring) 18(8):1670-1675(2010)
Tolson, K.P., et al. J. Neurosci. 30(10):3803-3812(2010)
Traurig, M., et al. Diabetes 58(7):1682-1689(2009)
Gregorio, S.P., et al. Psychiatry Res 165 (1-2), 1-9 (2009) :
Hung, C.C., et al. Int J Obes (Lond) 31(3):429-434(2007)

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¥ 1,100.00

Cat# AP12960A

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SIM1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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