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|Application ||WB, IHC-P, E|
|Calculated MW||20132 Da|
|Other Names||Bcl-2-related protein A1, Bcl-2-like protein 5, Bcl2-L-5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS, BCL2A1, BCL2L5, BFL1, GRS, HBPA1|
|Target/Specificity||This BCL2A1 (A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-65 amino acids from human BCL2A1 (A1).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BCL2A1 (A1) Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BCL2L5, BFL1, GRS, HBPA1|
|Function||Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity). Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (By similarity).|
|Tissue Location||Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies|
Provided below are standard protocols that you may find useful for product applications.
Bcl-2 related Protein A1 is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and has been shown to be up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival.
Akatsuka, Y., et al., J. Exp. Med. 197(11):1489-1500 (2003).
Edelstein, L.C., et al., Mol. Cell. Biol. 23(8):2749-2761 (2003).
Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002).
Akari, H., et al., J. Exp. Med. 194(9):1299-1311 (2001).
Harrington, J.J., et al., Nat. Biotechnol. 19(5):440-445 (2001).