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Bcl-2 Antibody (BH3 Domain Specific)

Purified Rabbit Polyclonal Antibody (Pab)

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Immunohistochemical analysis of AP1303a on paraffin-embedded Human placenta tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of AP1303a on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes : Anti-Bcl-2 Antibody (BH3 Domain Specific) at 1:1000 dilution Lane 1: RPMI 8226 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (Cat#AP1303a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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文献引用 : 31
实验流程
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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IF, WB, E
Primary Accession	P10415
Other Accession	O02718
Reactivity	Human, Rat, Mouse
Predicted	Human, Mouse, Bovine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	26266 Da
Antigen Region	75-110 aa

Additional Information
Gene ID	596
Other Names	Apoptosis regulator Bcl-2, BCL2
Target/Specificity	This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 amino acids from human Bcl.
Dilution	IHC-P~~1:100~500 WB~~1:1000 IF~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Bcl-2 Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	BCL2
Function	Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1- inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Cellular Location	Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Cytoplasm {ECO:0000250\|UniProtKB:P10417}
Tissue Location	Expressed in a variety of tissues.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.

REFERENCES

Zhu, C.J., et al., J. Neurosci. Res. 74(1):60-66 (2003). Scopa, C.D., et al., Dig. Dis. Sci. 48(10):1990-1997 (2003). Grace, V.M., et al., Gynecol. Oncol. 91(1):51-58 (2003). Thoulouze, M.I., et al., Virology 314(2):549-561 (2003). Iwata, A., et al., J. Immunol. 171(6):3136-3141 (2003).

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lin

2017-07-07 15:23:33

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Species tested

人

Application tested

检测细胞凋亡程度 IF 1:20 4°过夜二抗Anti Alexa Fluor 647（1;800）37° 1.5H DAPI 核染15min

Organization tested

HMrSV5细胞

Barcode encoding

Brief protocol

¥ 1,250.00

Cat# AP1303a

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

Quantity:

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Availability: In Stock

询价

Bcl-2 Antibody (BH3 Domain Specific)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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