癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NAE1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q13564 |
| Other Accession | Q9Z1A5, Q4R3L6, NP_001018170.1, NP_003896.1 |
| Reactivity | Human |
| Predicted | Monkey, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 60246 Da |
| Antigen Region | 172-200 aa |
| Gene ID | 8883 |
|---|---|
| Other Names | NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1 |
| Target/Specificity | This NAE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-200 amino acids from the Central region of human NAE1. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NAE1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NAE1 |
|---|---|
| Synonyms | APPBP1 |
| Function | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development. |
| Cellular Location | Cell membrane. Note=Colocalizes with APP in lipid rafts |
| Tissue Location | Ubiquitous in fetal tissues. Expressed throughout the adult brain. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.
REFERENCES
Jung, A.C., et al. Int. J. Cancer 126(8):1882-1894(2010)
Park, Y., et al. Biochem. Biophys. Res. Commun. 374(2):294-298(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Li, T., et al. Arch. Biochem. Biophys. 453(1):70-74(2006)
Norman, J.A., et al. Biochemistry 45(9):3014-3019(2006)
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