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Bok BH3 Domain Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Bok BH3 Domain Antibody AP1310a
    Western blot analysis of anti-Bok BH3 domain Pab (Cat. #AP1310a) in HL-60 cell lysate. Bok BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • 14 - Bok BH3 Domain Antibody AP1310a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9UMX3
Other Accession Q6KAQ7, Q8IYH5, Q5SSH7, O43149, A5H447, Q62523, Q15942, Q04584, G5EEM5, Q9GT24, A0JMZ3, Q5TYQ1, P21541, Q3UFS0, Q9C0D3, A2BFL2, Q6WRX3, Q8C8V1, Q2QGD7, P98169, P98168, A2CE44, Q8VIL3, Q9CQU5, O95229, Q2TBH8, Q6IRM9, Q8R060, Q9H900, Q9VA00, A5WWB6, Q95XP9
Reactivity Human
Predicted Drosophila, C.Elegans, Bovine, Chicken, Human, Monkey, Mouse, Rat, Rabbit, Xenopus, Zebrafish, Yeast, Pig, Vaccinia Virus, Hamster, Neisseria Gonorrhoeae, SARS
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 23280 Da
Additional Information
Gene ID 666
Other Names Bcl-2-related ovarian killer protein, hBOK, Bcl-2-like protein 9, Bcl2-L-9, BOK, BCL2L9
Dilution WB~~1:1000
IHC-P~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBok BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BOK (HGNC:1087)
Synonyms BCL2L9
Function [Isoform 1]: Apoptosis regulator that functions through different apoptotic signaling pathways (PubMed:27076518, PubMed:15102863, PubMed:20673843). Plays a roles as pro-apoptotic protein that positively regulates intrinsic apoptotic process in a BAX- and BAK1-dependent manner or in a BAX- and BAK1-independent manner (PubMed:27076518, PubMed:15102863). In response to endoplasmic reticulum stress promotes mitochondrial apoptosis through downstream BAX/BAK1 activation and positive regulation of PERK-mediated unfolded protein response (By similarity). Activates apoptosis independently of heterodimerization with survival-promoting BCL2 and BCL2L1 through induction of mitochondrial outer membrane permeabilization, in a BAX- and BAK1-independent manner, in response to inhibition of ERAD- proteasome degradation system, resulting in cytochrome c release (PubMed:27076518). In response to DNA damage, mediates intrinsic apoptotic process in a TP53-dependent manner (PubMed:15102863). Plays a role in granulosa cell apoptosis by CASP3 activation (PubMed:20673843). Plays a roles as anti-apoptotic protein during neuronal apoptotic process, by negatively regulating poly ADP-ribose polymerase-dependent cell death through regulation of neuronal calcium homeostasis and mitochondrial bioenergetics in response to NMDA excitation (By similarity). In addition to its role in apoptosis, may regulate trophoblast cell proliferation during the early stages of placental development, by acting on G1/S transition through regulation of CCNE1 expression (PubMed:19942931). May also play a role as an inducer of autophagy by disrupting interaction between MCL1 and BECN1 (PubMed:24113155).
Cellular Location [Isoform 1]: Mitochondrion membrane {ECO:0000250|UniProtKB:O35425}; Single-pass membrane protein {ECO:0000250|UniProtKB:O35425}. Endoplasmic reticulum membrane; Single-pass membrane protein {ECO:0000250|UniProtKB:O35425}. Mitochondrion inner membrane. Cytoplasm. Nucleus. Mitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane. Early endosome membrane {ECO:0000250|UniProtKB:O35425}. Recycling endosome membrane {ECO:0000250|UniProtKB:O35425}. Nucleus outer membrane {ECO:0000250|UniProtKB:O35425}. Golgi apparatus, cis-Golgi network membrane {ECO:0000250|UniProtKB:O35425}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:O35425}. Membrane. Note=Nuclear and cytoplasmic compartments in the early stages of apoptosis and during apoptosis it associates with mitochondria (PubMed:19942931). In healthy cells, associates loosely with the membrane in a hit-and-run mode. The insertion and accumulation on membranes is enhanced through the activity of death signals, resulting in the integration of the membrane-bound protein into the membrane (PubMed:15868100). The transmembrane domain controls subcellular localization; constitutes a tail-anchor. Localizes in early and late endosome upon blocking of apoptosis. Must localize to the mitochondria to induce mitochondrial outer membrane permeabilization and apoptosis (By similarity) {ECO:0000250|UniProtKB:O35425, ECO:0000269|PubMed:15868100, ECO:0000269|PubMed:19942931}
Tissue Location Expressed mainly in oocytes; weak expression in granulosa cells of the developing follicles. In adult human ovaries, expressed in granulosa cells at all follicular stages, but expression in primordial/primary follicles granulosa cell is stronger than in secondary and antral follicles.
Research Areas

BACKGROUND

Bok belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains all four BCL-2 like domains (BH1, 2, 3 and 4) and is a pro-apoptotic BCL-2 protein identified in the ovary.

REFERENCES

Hsu, S.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 94(23):12401-12406 (1997). Zhang, H., et al., FEBS Lett. 480 (2-3), 311-313 (2000).

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