癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ULK3 Antibody (C-term) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, E |
|---|---|
| Primary Accession | Q6PHR2 |
| Other Accession | NP_001092906.1 |
| Reactivity | Mouse |
| Predicted | Rat, Bovine, Canine, Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 53444 Da |
| Antigen Region | 347-375 aa |
| Gene ID | 25989 |
|---|---|
| Other Names | Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3 |
| Target/Specificity | This ULK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 347-375 amino acids from the C-terminal region of human ULK3. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ULK3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ULK3 {ECO:0000303|PubMed:19878745, ECO:0000312|HGNC:HGNC:19703} |
|---|---|
| Function | Serine/threonine protein kinase that acts as a regulator of smoothened signaling and autophagy (PubMed:19279323, PubMed:19878745, PubMed:20643644, PubMed:31279575). Acts as a positive regulator of smoothened signaling in the presence of hedgehog (DHH, IHH or SHH) ligand: autophosphorylates and mediates phosphorylation of full-length GLI2, promoting subsequent phosphorylation by CDK1 and GLI2 dissociation from SUFU and translocation to the nucleus (PubMed:19878745, PubMed:20643644, PubMed:31279575). Also phosphorylates as GLI1 and GLI3, although less efficiently (PubMed:19878745, PubMed:20643644). In the absence of hedgehog ligand, interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3) (PubMed:20643644). Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy (PubMed:19279323). |
| Cellular Location | Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence. |
| Tissue Location | Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
REFERENCES
Maloverjan, A., et al. J. Biol. Chem. 285(39):30079-30090(2010)
Takeuchi, F., et al. Circulation 121(21):2302-2309(2010)
Maloverjan, A., et al. Exp. Cell Res. 316(4):627-637(2010)
Levy, D., et al. Nat. Genet. 41(6):677-687(2009)
Kim, D.H., et al. Blood 113(11):2517-2525(2009)
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