注册 | 登录
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   VPS4B Antibody (C-term)   

VPS4B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - VPS4B Antibody (C-term) AP13813b
    VPS4B Antibody (C-term) (Cat. #AP13813b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the VPS4B antibody detected the VPS4B protein (arrow).
  • 产品详情
  • 文献引用 : 1
  • 实验流程
  • 背景知识
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O75351
Other Accession NP_004860.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49302 Da
Additional Information
Gene ID 9525
Other Names Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42
Target/Specificity This VPS4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-332 amino acids from the C-terminal region of human VPS4B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVPS4B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS4B (HGNC:10895)
Synonyms SKD1, VPS42
Function Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission (PubMed:18687924). Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Cellular Location Late endosome membrane {ECO:0000250|UniProtKB:P46467}; Peripheral membrane protein. Note=Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
Tissue Location Ubiquitously expressed.
Research Areas

BACKGROUND

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq].

REFERENCES

Morita, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894(2010)
McDonough, C.W., et al. Hum. Genet. (2009) In press :
Bruce, E.A., et al. Virology 390(2):268-278(2009)
Inoue, M., et al. Traffic 9(12):2180-2189(2008)
Stuchell-Brereton, M.D., et al. Nature 449(7163):740-744(2007)

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价