EFCB7 Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

EFCB7 Antibody (C-term) (Cat. #AP14097b) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the EFCB7 antibody detected the EFCB7 protein (arrow).
EFCB7 Antibody (C-term) (Cat. #AP14097b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EFCB7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
EFCB7 Antibody (C-term) (Cat. #AP14097b) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	A8K855
Other Accession	NP_115813.2
Reactivity	Human, Mouse
Predicted	Rat, Bovine, Canine, Rabbit
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	71981 Da
Antigen Region	434-462 aa

Additional Information
Gene ID	84455
Other Names	EF-hand calcium-binding domain-containing protein 7, EFCAB7, KIAA1799
Target/Specificity	This EFCB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 434-462 amino acids from the C-terminal region of human EFCB7.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	EFCB7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	EFCAB7
Synonyms	KIAA1799
Function	Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling. Required for the localization of the EVC2:EVC subcomplex at the base of primary cilia.
Cellular Location	Cell projection, cilium membrane {ECO:0000250\|UniProtKB:Q8VDY4}; Peripheral membrane protein {ECO:0000250\|UniProtKB:Q8VDY4}; Cytoplasmic side {ECO:0000250\|UniProtKB:Q8VDY4}. Note=The EvC complex localizes at the base of cilia in the EvC zone of primary cilia in a EFCAB7-dependent manner. {ECO:0000250\|UniProtKB:Q8VDY4}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.