癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DUT Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P33316 |
| Other Accession | P70583, NP_001939.1, NP_001020419.1, NP_001020420.1 |
| Reactivity | Human |
| Predicted | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 26563 Da |
| Antigen Region | 170-198 aa |
| Gene ID | 1854 |
|---|---|
| Other Names | Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, DUT |
| Target/Specificity | This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT. |
| Dilution | IHC-P~~1:100~500 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DUT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DUT |
|---|---|
| Function | Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis (PubMed:17880943, PubMed:8631816, PubMed:8805593). Inhibits peroxisome proliferator- activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor (By similarity). Essential for embryonic development (By similarity). |
| Cellular Location | [Isoform 2]: Nucleus |
| Tissue Location | Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level) |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
REFERENCES
Takatori, H., et al. Liver Int. 30(3):438-446(2010) Quesada-Soriano, I., et al. Biochimie 92(2):178-186(2010) Chanson, A., et al. Am. J. Clin. Nutr. 89(6):1927-1936(2009) Takacs, E., et al. FEBS Lett. 583(5):865-871(2009) Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
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