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>   首页   >   产品   >   一抗   >   癌症   >   E Cadherin (CDH1) Antibody (N-term)   

E Cadherin (CDH1) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - E Cadherin (CDH1) Antibody (N-term) AP1477a
    All lanes : Anti-CDH1 Antibody (N-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: DU145 whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - E Cadherin (CDH1) Antibody (N-term) AP1477a
    Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P12830
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 97456 Da
Antigen Region 160-189 aa
Additional Information
Gene ID 999
Other Names Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO
Target/Specificity This E Cadherin (CDH1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1).
Dilution WB~~1:2000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsE Cadherin (CDH1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDH1 (HGNC:1748)
Function Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cellular Location Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Cytoplasm {ECO:0000250|UniProtKB:P09803}. Cell junction {ECO:0000250|UniProtKB:Q90Z37}. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane
Tissue Location Expressed in granuloma macrophages (at protein level) (PubMed:27760340). Expressed in the liver (PubMed:3263290)
Research Areas

BACKGROUND

CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.

REFERENCES

Mansouri,A., Differentiation 38 (1), 67-71 (1988) Knudsen,K.A. J. Cell Biol. 118 (3), 671-679 (1992) Hsu,Y.M., Cancer Res. 67 (22), 11064-11073 (2007)

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