- 文献引用 : 5
|Application ||FC, WB, E|
|Calculated MW||97456 Da|
|Other Names||Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO|
|Target/Specificity||This E Cadherin (CDH1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1).|
|Format||Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||E Cadherin (CDH1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.|
|Cellular Location||Cell junction. Cell membrane; Single-pass type I membrane protein Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane|
|Tissue Location||Non-neural epithelial tissues.|
Author : Pan L1,Yang H1,Xu C1,Chen S1,Meng Z2,Li K3,Chen H4.
Biomed Pharmacother. 2018 Sep;105:566-572. doi: 10.1016/j.biopha.2018.06.001. Epub 2018 Jun 8.
Author : Wang K1,2,Qu X1,Liu S1,Yang X1,Bie F1,Wang Y1,Huang C1,Du J3,4.
J Cancer Res Clin Oncol. 2018 May 4. doi: 10.1007/s00432-018-2653-1. [Epub ahead of print]
Author : Dai Y1, Duan H2, Duan C3, Zhou R1, He Y1, Tu Q1, Shen L4.
Biochem Biophys Res Commun. 2015 Oct 1. pii: S0006-291X(15)30626-4. doi: 10.1016/j.bbrc.2015.09.109. [Epub ahead of print]
Author : Liu XB1, Li F1, Li YQ1, Yang F1.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):10887-93. eCollection 2015.
Author : Hu P1, Wang G, Shen M, Zhang P, Zhang J, Du J, Liu Q.
Future Oncol. 2015 Mar;11(5):771-83. doi: 10.2217/fon.14.306.
Provided below are standard protocols that you may find useful for product applications.
CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.
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