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Calmodulin Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

AP1570b staining hCalmodulin-D132 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AP1570b staining hCalmodulin-D132 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-Calmodulin Antibody at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Mouse brain lysate Lane 4: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-Calmodulin Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-hCalmodulin-D132 at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-Calmodulin Antibody (C-term)(Cat.#AP1570b) in Hela cell line lysates (35ug/lane). Calmodulin(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue reacted with Calmodulin antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	P62158
Other Accession	P62155, P62161, P62160, P62204, Q6PI52, P62149, P62157
Reactivity	Human, Rat, Mouse
Predicted	Bovine, Chicken, Zebrafish, Mouse, Rabbit, Rat, Xenopus
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	117-149 aa

Additional Information
Other Names	Calmodulin, CaM, CALM1, CALM, CAM, CAM1
Target/Specificity	This Calmodulin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-149 amino acids from the C-terminal region of human Calmodulin.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Calmodulin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

REFERENCES

Radding, W., et al., AIDS Res. Hum. Retroviruses 16(15):1519-1525 (2000).
Wang, D., et al., J. Neurochem. 75(2):763-771 (2000).
Toutenhoofd, S.L., et al., Cell Calcium 23(5):323-338 (1998).
Matoba, R., et al., Gene 146(2):199-207 (1994).
Berchtold, M.W., et al., Genomics 16(2):461-465 (1993).

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¥ 1,250.00

Cat# AP1570b

Size:

50 µl

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¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

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询价

Calmodulin Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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