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GIPC1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Immunohistochemical analysis of paraffin-embedded human kidney tissue using AP16086a performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Overlay histogram showing U-2 OS cells stained with AP16086a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP16086a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
GIPC1 Antibody (N-term) (Cat. #AP16086a) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the GIPC1 antibody detected the GIPC1 protein (arrow).
GIPC1 Antibody (N-term) (Cat. #AP16086a) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the GIPC1 antibody detected the GIPC1 protein (arrow).

产品详情
文献引用 : 1
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P-Leica, E
Primary Accession	O14908
Other Accession	Q9Z254, Q9Z0G0, NP_974199.1, NP_974196.1
Reactivity	Human, Mouse
Predicted	Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	36049 Da
Antigen Region	52-81 aa

Additional Information
Gene ID	10755
Other Names	PDZ domain-containing protein GIPC1, GAIP C-terminus-interacting protein, RGS-GAIP-interacting protein, RGS19-interacting protein 1, Synectin, Tax interaction protein 2, TIP-2, GIPC1, C19orf3, GIPC, RGS19IP1
Target/Specificity	This GIPC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human GIPC1.
Dilution	WB~~1:1000 FC~~1:25 IHC-P-Leica~~1:500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GIPC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GIPC1
Synonyms	C19orf3, GIPC, RGS19IP1
Function	May be involved in G protein-linked signaling.
Cellular Location	Cytoplasm. Membrane; Peripheral membrane protein
Tissue Location	Widely expressed (PubMed:9770488). Expressed in skeletal muscle (at protein level) (PubMed:32413282)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

GIPC1 is a scaffolding protein that regulates cell surface receptor expression and trafficking (Lee et al., 2008 [PubMed 18775991]).

REFERENCES

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wu, D., et al. J. Biol. Chem. 285(37):28643-28650(2010)
Razanskas, R., et al. Arch. Virol. 155(2):247-250(2010)
Puri, C., et al. Oncogene 29(2):188-200(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

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¥ 1,250.00

Cat# AP16086a

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

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GIPC1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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