癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DIAPH3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q9NSV4 |
| Other Accession | NP_001035982.1, NP_112194.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 136926 Da |
| Antigen Region | 1061-1090 aa |
| Gene ID | 81624 |
|---|---|
| Other Names | Protein diaphanous homolog 3, Diaphanous-related formin-3, DRF3, MDia2, DIAPH3, DIAP3 |
| Target/Specificity | This DIAPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1090 amino acids from the C-terminal region of human DIAPH3. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DIAPH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DIAPH3 |
|---|---|
| Synonyms | DIAP3 |
| Function | Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity. |
| Cellular Location | Cytoplasm. Nucleus {ECO:0000250|UniProtKB:Q9Z207} Note=During mitosis, co-localizes with the actin-rich cleavage furrow and with the microtubule-rich central spindle during cytokinesis (PubMed:18755006, PubMed:19457867). Shuttles between the cytoplasm and the nucleus (By similarity). {ECO:0000250|UniProtKB:Q9Z207, ECO:0000269|PubMed:18755006, ECO:0000269|PubMed:19457867} |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).
REFERENCES
DeWard, A.D., et al. J. Biol. Chem. 284(30):20061-20069(2009)
Block, J., et al. J Microsc 231(3):506-517(2008)
Beli, P., et al. Nat. Cell Biol. 10(7):849-857(2008)
Yang, C., et al. PLoS Biol. 5 (11), E317 (2007) :
Gupton, S.L., et al. J. Cell. Sci. 120 (PT 19), 3475-3487 (2007) :
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