癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
P4HA1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | P13674 |
| Other Accession | Q1RMU3, NP_001136067.1, NP_000908.2, NP_001017962.1 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 61049 Da |
| Antigen Region | 414-442 aa |
| Gene ID | 5033 |
|---|---|
| Other Names | Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1, Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA |
| Target/Specificity | This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | P4HA1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | P4HA1 |
|---|---|
| Synonyms | P4HA |
| Function | Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. |
| Cellular Location | Endoplasmic reticulum lumen. |
| Tissue Location | Expressed in the heart, liver, skeletal muscle, kidney, placenta, lung and pancreas. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
REFERENCES
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Gorres, K.L., et al. PLoS ONE 4 (11), E7635 (2009) :
Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006)
Fahling, M., et al. J. Biol. Chem. 281(36):26089-26101(2006)
Grimmer, C., et al. Am. J. Pathol. 169(2):491-502(2006)
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Anonymous
2016-08-24 09:20:44
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Species tested
Human
Application tested
WB
Organization tested
U87,LO2,A431,HT1080,MCF-7
Barcode encoding
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose
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