癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ARF5 Antibody (Center) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | P84085 |
| Other Accession | P84083, P84084, NP_001653.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat, Bovine, Canine, Rabbit, Chicken |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20530 Da |
| Antigen Region | 80-108 aa |
| Gene ID | 381 |
|---|---|
| Other Names | ADP-ribosylation factor 5, ARF5 |
| Target/Specificity | This ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the Central region of human ARF5. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ARF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ARF5 |
|---|---|
| Function | GTP-binding protein involved in protein trafficking. Contributes to vesicle budding and uncoating at the Golgi apparatus. Regulates mTORC1 activity at the plasma membrane by promoting its recruitment to membrane ruffles, facilitating activation via the PI3K/Akt signaling pathway (PubMed:36735494). Acts as an early responder to lysosomal damage and participates in lysosomal quality control pathways (PubMed:41293316). |
| Cellular Location | Cell membrane. Golgi apparatus. Cytoplasm, perinuclear region. Lysosome membrane; Lipid-anchor. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor {ECO:0000250|UniProtKB:P84084} |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq].
REFERENCES
Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(42):15416-15421(2006)
Austin, C., et al. Biochemistry 41(14):4669-4677(2002)
Shin, O.H., et al. Biochemistry 40(36):10846-10852(2001)
Nevrivy, D.J., et al. J. Biol. Chem. 275(22):16827-16836(2000)
Kondo, A., et al. Mol. Biol. Cell 11(4):1315-1327(2000)
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