PRRT2 Antibody (Center) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Overlay histogram showing U-2 OS cells stained with AP17642c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP17642c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
PRRT2 Antibody (Center) (Cat. #AP17642c) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PRRT2 antibody detected the PRRT2 protein (arrow).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q7Z6L0
Other Accession	NP_660282.2
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	34945 Da
Antigen Region	207-233 aa

Additional Information
Gene ID	112476
Other Names	Proline-rich transmembrane protein 2, Dispanin subfamily B member 3, DSPB3, PRRT2
Target/Specificity	This PRRT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-233 amino acids from the Central region of human PRRT2.
Dilution	WB~~1:1000 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PRRT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PRRT2
Function	As a component of the outer core of AMPAR complex, may be involved in synaptic transmission in the central nervous system. In hippocampal neurons, in presynaptic terminals, plays an important role in the final steps of neurotransmitter release, possibly by regulating Ca(2+)-sensing. In the cerebellum, may inhibit SNARE complex formation and down-regulate short-term facilitation.
Cellular Location	Cell membrane; Single-pass membrane protein {ECO:0000250\|UniProtKB:E9PUL5}. Presynaptic cell membrane {ECO:0000250\|UniProtKB:E9PUL5}; Single-pass membrane protein {ECO:0000250\|UniProtKB:E9PUL5}. Synapse {ECO:0000250\|UniProtKB:E9PUL5} Cell projection, axon. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250\|UniProtKB:D3ZFB6}. Postsynaptic density membrane {ECO:0000250\|UniProtKB:D3ZFB6}. Cell projection, dendritic spine {ECO:0000250\|UniProtKB:D3ZFB6}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.