癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GCLC Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | P48506 |
| Other Accession | P19468, P97494, NP_001489.1 |
| Reactivity | Human, Mouse |
| Predicted | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 72766 Da |
| Antigen Region | 286-315 aa |
| Gene ID | 2729 |
|---|---|
| Other Names | Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, GLCLC |
| Target/Specificity | This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human GCLC. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GCLC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GCLC (HGNC:4311) |
|---|---|
| Synonyms | GLCL, GLCLC |
| Function | Catalyzes the ATP-dependent ligation of L-glutamate and L- cysteine and participates in the first and rate-limiting step in glutathione biosynthesis. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
REFERENCES
Man, B.L., et al. J Clin Neurosci 17(10):1244-1247(2010)
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010)
Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010)
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :
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