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>   首页   >   产品   >   一抗   >   精选抗体   >   细胞自噬抗体   >   PI3KC3 Antibody (N-term G24)   

PI3KC3 Antibody (N-term G24)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PI3KC3 Antibody (N-term G24) AP1851a
    Western blot analysis of PI3KC3 Antibody (N-term G24)(Cat. #AP8014a) in HeLa cell lysate. PI3KC3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • 14 - PI3KC3 Antibody (N-term G24) AP1851a
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (N-term) (Cat.#AP1851a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - PI3KC3 Antibody (N-term G24) AP1851a
    Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term G24)(Cat#AP1851a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q8NEB9
Other Accession Q6AZN6, O88763, Q5D891, Q6PF93
Reactivity Human
Predicted Mouse, Pig, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 101549 Da
Additional Information
Gene ID 5289
Other Names Phosphatidylinositol 3-kinase catalytic subunit type 3, PI3-kinase type 3, PI3K type 3, PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34, PIK3C3, VPS34
Target/Specificity This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-41 amino acids from the N-terminal region of human PI3KC3.
Dilution WB~~1:1000
IHC-P~~1:100~500
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI3KC3 Antibody (N-term G24) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIK3C3 (HGNC:8974)
Synonyms VPS34 {ECO:0000305}
Function Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:14617358, PubMed:7628435, PubMed:33637724). As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding (PubMed:32690950). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123). Involved in the transport of lysosomal enzyme precursors to lysosomes (By similarity). Required for transport from early to late endosomes (By similarity).
Cellular Location Midbody. Late endosome. Cytoplasmic vesicle, autophagosome. Note=As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (PubMed:14617358). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity) {ECO:0000250|UniProtKB:Q6PF93, ECO:0000305|PubMed:14617358}
Tissue Location Ubiquitously expressed, with a highest expression in skeletal muscle.
Research Areas

BACKGROUND

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

REFERENCES

Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003).
Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).

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