癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ALG8 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, E |
|---|---|
| Primary Accession | Q9BVK2 |
| Other Accession | Q6P8H8, Q0P5D9, NP_076984.2 |
| Reactivity | Human |
| Predicted | Bovine, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 60088 Da |
| Antigen Region | 15-41 aa |
| Gene ID | 79053 |
|---|---|
| Other Names | Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1, 3-glucosyltransferase, Asparagine-linked glycosylation protein 8 homolog, Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1, 3-glucosyltransferase, Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase, ALG8 |
| Target/Specificity | This ALG8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-41 amino acids from the N-terminal region of human ALG8. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ALG8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ALG8 {ECO:0000303|PubMed:28375157, ECO:0000312|HGNC:HGNC:23161} |
|---|---|
| Function | Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3- glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the second glucose residue from dolichyl phosphate glucose (Dol-P- Glc) onto the lipid-linked oligosaccharide intermediate Glc(1)Man(9)GlcNAc(2)-PP-Dol to produce Glc(2)Man(9)GlcNAc(2)-PP-Dol. Glc(2)Man(9)GlcNAc(2)-PP-Dol is a substrate for ALG10, the following enzyme in the biosynthetic pathway (PubMed:12480927, PubMed:15235028). Required for PKD1/Polycystin-1 maturation and localization to the plasma membrane of the primary cilia (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a member of the ALG6/ALG8 glucosyltransferase family. The encoded protein catalyzes the addition of the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation of proteins. Mutations in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ih). Alternatively spliced transcript variants encoding different isoforms have been identified.
REFERENCES
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Stolting, T., et al. Mol. Genet. Metab. 98(3):305-309(2009)
Jaeken, J., et al. Curr. Opin. Pediatr. 16(4):434-439(2004)
Schollen, E., et al. J. Med. Genet. 41(7):550-556(2004)
Jaeken, J. J. Inherit. Metab. Dis. 27(3):423-426(2004)
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