癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
B4GALT4 Antibody (Center) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | O60513 |
| Other Accession | NP_003769.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 40041 Da |
| Antigen Region | 91-117 aa |
| Gene ID | 8702 |
|---|---|
| Other Names | Beta-1, 4-galactosyltransferase 4, Beta-1, 4-GalTase 4, Beta4Gal-T4, b4Gal-T4, 241-, UDP-Gal:beta-GlcNAc beta-1, 4-galactosyltransferase 4, UDP-galactose:beta-N-acetylglucosamine beta-1, 4-galactosyltransferase 4, N-acetyllactosamine synthase, Nal synthase, Lactotriaosylceramide beta-1, 4-galactosyltransferase, Beta-N-acetylglucosaminyl-glycolipid beta-1, 4-galactosyltransferase, B4GALT4 |
| Target/Specificity | This B4GALT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-117 amino acids from the Central region of human B4GALT4. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | B4GALT4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | B4GALT4 {ECO:0000303|PubMed:17690104, ECO:0000312|HGNC:HGNC:927} |
|---|---|
| Function | Galactose (Gal) transferase involved in the synthesis of terminal N-acetyllactosamine (LacNac) unit present on glycan chains of glycoproteins and glycosphingolipids (PubMed:12511560, PubMed:17690104, PubMed:32827291, PubMed:9792633). Catalyzes the transfer of Gal residue via a beta1->4 linkage from UDP-Gal to the non-reducing terminal N- acetyl glucosamine 6-O-sulfate (6-O-sulfoGlcNAc) in the linearly growing chain of both N- and O-linked keratan sulfate proteoglycans. Cooperates with B3GNT7 N-acetyl glucosamine transferase and CHST6 and CHST1 sulfotransferases to construct and elongate mono- and disulfated disaccharide units [->3Galbeta1->4(6-sulfoGlcNAcbeta)1->] and [->3(6- sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed:17690104). Transfers Gal residue via a beta1->4 linkage to terminal 6-O-sulfoGlcNAc within the LacNac unit of core 2 O-glycans forming 6-sulfo-sialyl-Lewis X (sLex). May contribute to the generation of sLex epitope on mucin-type glycoproteins that serve as ligands for SELL/L-selectin, a major regulator of leukocyte migration (PubMed:12511560). In the biosynthesis pathway of neolacto-series glycosphingolipids, transfers Gal residue via a beta1->4 linkage to terminal GlcNAc of a lactotriaosylceramide (Lc3Cer) acceptor to form a neolactotetraosylceramide (PubMed:9792633). Efficiently galactosylates both non-sulfated and 6-O-sulfated terminal GlcNAc moities on G0 complex-type N-glycans. |
| Cellular Location | Golgi apparatus membrane; Single-pass type II membrane protein. Secreted |
| Tissue Location | Highest expression is observed in placenta, pancreas, kidney and heart (PubMed:9792633). Expressed in corneal epithelial cells (PubMed:17690104). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.
REFERENCES
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Guo, S., et al. Glycobiology 11(10):813-820(2001)
Suzuki, Y., et al. Genome Res. 11(5):677-684(2001)
Amado, M., et al. Biochim. Biophys. Acta 1473(1):35-53(1999)
Fan, Y., et al. Sci. China, C, Life Sci. 42(4):337-345(1999)
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