B7H6 Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-B7H6 Antibody (C-term) at 1:500-1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: Molt-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 53kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-B7H6 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: Hela whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-B7H6 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: Hela whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysate from K562 cell line, using B7H6 Antibody (C-term)(Cat. #AP18769b). AP18769b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
B7H6 Antibody (C-term)(Cat. #AP18769b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the B7H6 antibody detected the B7H6 protein (arrow).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q68D85
Other Accession	NP_001189368.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	50827 Da
Antigen Region	389-415 aa

Additional Information
Gene ID	374383
Other Names	Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7-H6, NCR3LG1, B7H6
Target/Specificity	This B7H6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-415 amino acids from the C-terminal region of human B7H6.
Dilution	WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	B7H6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NCR3LG1
Synonyms	B7H6
Function	Triggers NCR3-dependent natural killer cell activation.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen- transformed cells (at protein level).

Research Areas