|Application ||WB, E|
|Calculated MW||60348 Da|
|Other Names||Heat shock factor protein 2, HSF 2, Heat shock transcription factor 2, HSTF 2, HSF2, HSTF2|
|Target/Specificity||This HSF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the Central region of human HSF2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HSF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.|
|Cellular Location||Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation|
Provided below are standard protocols that you may find useful for product applications.
HSF2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Although the names HSF1 and HSF2 were chosen for historical reasons, these peptides should be referred to as heat-shock transcription factors.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Xing, H., et al. Cell Stress Chaperones 15(3):301-308(2010)
Sandqvist, A., et al. Mol. Biol. Cell 20(5):1340-1347(2009)
Tateishi, Y., et al. J. Biol. Chem. 284(4):2435-2447(2009)
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)