MBD6 Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-MBD6 Antibody (C-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
MBD6 Antibody (C-term) (Cat. #AP18913b) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MBD6 antibody detected the MBD6 protein (arrow).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q96DN6
Other Accession	Q3TY92, NP_443129.3
Reactivity	Human, Mouse
Predicted	Rat, Bovine, Canine, Rabbit
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	101201 Da
Antigen Region	977-1003 aa

Additional Information
Gene ID	114785
Other Names	Methyl-CpG-binding domain protein 6, Methyl-CpG-binding protein MBD6, MBD6, KIAA1887
Target/Specificity	This MBD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 977-1003 amino acids from the C-terminal region of human MBD6.
Dilution	WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MBD6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MBD6
Synonyms	KIAA1887
Function	Non-catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed:24634419). Important for stability of PR-DUB components and stimulating its ubiquitinase activity (PubMed:36180891). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed:36180891). MBD5 and MBD6 containing complexes associate with distinct chromatin regions enriched in genes involved in different pathways (PubMed:36180891). Heterochromatin recruitment is not mediated by DNA methylation (PubMed:20700456). The PR-DUB complex is an epigenetic regulator of gene expression, including genes involved in development, cell communication, signaling, cell proliferation and cell viability; may promote cancer cell growth (PubMed:36180891).
Cellular Location	Nucleus. Chromosome. Note=Associates with heterochromatin containing chromocentres in a subset of cells in a DNA methylation- independent manner (PubMed:20700456). Localizes to sites of DNA damage independent of the PR-DUB complex (PubMed:24634419)