癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
G2E3 Antibody (Center) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q7L622 |
| Other Accession | Q4R9C4, NP_060239.2 |
| Reactivity | Human |
| Predicted | Monkey, Bovine, Canine, Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 80504 Da |
| Antigen Region | 226-252 aa |
| Gene ID | 55632 |
|---|---|
| Other Names | G2/M phase-specific E3 ubiquitin-protein ligase, 632-, G2E3, KIAA1333 |
| Target/Specificity | This G2E3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-252 amino acids from the Central region of human G2E3. |
| Dilution | WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | G2E3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | G2E3 {ECO:0000303|PubMed:17239372, ECO:0000312|HGNC:HGNC:20338} |
|---|---|
| Function | E3 ubiquitin-protein ligase involved in the establishment of constitutive heterochromatin at pericentric regions by catalyzing histone H3 'Lys-14' monoubiquitination (H3K14ub) (PubMed:41094145). H3K14ub represents a specific tag for heterochromatin formation by facilitating the deposition of histone H3 trimethylated at 'Lys-9' (H3K9me3) (PubMed:41094145). Highly expressed in G2/M phase and associates with mitotic chromosomes in an RNA-dependent manner to catalyze H3K14ub, thereby promoting recruitment of SUV39H (SUV39H1 and/or SUV39H2) and subsequent recruitment of HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones (PubMed:41094145). |
| Cellular Location | Nucleus. Chromosome Nucleus, nucleolus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm (PubMed:17239372). In the nucleus, delocalizes from the nucleolus to the nucleoplasm in response to DNA damage (PubMed:17239372). Associates with mitotic chromosomes; enriched at pericentromeric heterochromatin (PubMed:41094145) |
| Tissue Location | Predominantly expressed in brain, liver, kidney, testes and ovary. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.
REFERENCES
Brooks, W.S., et al. J. Biol. Chem. 283(32):22304-22315(2008)
Brooks, W.S., et al. Exp. Cell Res. 313(4):665-676(2007)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Crawford, D.F., et al. J. Biol. Chem. 276(40):37166-37177(2001)
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