癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SPAM1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | P38567 |
| Other Accession | P38568, NP_694859.1 |
| Reactivity | Human |
| Predicted | Monkey |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57848 Da |
| Antigen Region | 429-457 aa |
| Gene ID | 6677 |
|---|---|
| Other Names | Hyaluronidase PH-20, Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, SPAM1, HYAL3, PH20 |
| Target/Specificity | This SPAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-457 amino acids from the C-terminal region of human SPAM1. |
| Dilution | WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SPAM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPAM1 |
|---|---|
| Synonyms | HYAL3, PH20 |
| Function | Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. |
| Cellular Location | Cell membrane; Lipid-anchor, GPI-anchor. |
| Tissue Location | Testis.. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
REFERENCES
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Dunn, C.A., et al. BMC Genomics 6 (1), 47 (2005) :
Evans, E.A., et al. Reprod. Biol. Endocrinol. 1, 54 (2003) :
Cherr, G.N., et al. Matrix Biol. 20(8):515-525(2001)
Csoka, A.B., et al. Matrix Biol. 20(8):499-508(2001)
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