癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PSMD14 Antibody (C-term) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | O00487 |
| Other Accession | O35593, NP_005796.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat, Bovine, Canine, Rabbit, Chicken |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 34577 Da |
| Antigen Region | 258-287 aa |
| Gene ID | 10213 |
|---|---|
| Other Names | 26S proteasome non-ATPase regulatory subunit 14, 3419-, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1, PSMD14, POH1 |
| Target/Specificity | This PSMD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from the C-terminal region of human PSMD14. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PSMD14 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PSMD14 |
|---|---|
| Synonyms | POH1 |
| Function | Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed:9374539, PubMed:1317798). The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex (PubMed:22909820). Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation (PubMed:22909820). Also involved in homologous recombination repair by promoting RAD51 loading (PubMed:22909820). Regulates macroautophagy by ensuring Golgi-to-ER retrograde transport through its deubiquitinating activity on K63-linked ubiquitin chains. This activity prevents the retention of essential autophagy proteins at the Golgi, enabling their trafficking to autophagosome formation sites and supporting Golgi-ER membrane recycling critical for effective autophagy (PubMed:32210007). |
| Tissue Location | Widely expressed. Highest levels in heart and skeletal muscle. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PSMD14 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]).
REFERENCES
Byrne, A., et al. Exp. Cell Res. 316(2):258-271(2010)
Ma, Z., et al. Pediatr Int 51(5):732-735(2009)
Cooper, E.M., et al. EMBO J. 28(6):621-631(2009)
Koulich, E., et al. Mol. Biol. Cell 19(3):1072-1082(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
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