|Application ||WB, E|
|Calculated MW||34555 Da|
|Other Names||TIMELESS-interacting protein, TIPIN|
|Target/Specificity||This TIPIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-254 amino acids from the N-terminal region of human TIPIN.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TIPIN Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays an important role in the control of DNA replication and the maintenance of replication fork stability. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress.|
|Cellular Location||Cytoplasm. Nucleus|
Provided below are standard protocols that you may find useful for product applications.
Required for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.
Kemp, M.G., et al. J. Biol. Chem. 285(22):16562-16571(2010)
Leman, A.R., et al. J. Cell. Sci. 123 (PT 5), 660-670 (2010) :
Utge, S.J., et al. PLoS ONE 5 (2), E9259 (2010) :
Unsal-Kacmaz, K., et al. Mol. Cell. Biol. 27(8):3131-3142(2007)
Gotter, A.L., et al. J. Mol. Biol. 366(1):36-52(2007)