Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 文献引用 : 1
|Application ||WB, E|
|Other Accession||P57053, Q64524, Q8CGP1, Q2PFX4, O60814, Q2M2T1, P06900, P02281, NP_066402.2|
|Predicted||Xenopus, Bovine, Monkey, Mouse|
|Calculated MW||13904 Da|
|Other Names||Histone H2B type 1-J, Histone H2B1, Histone H2Br, H2B/r, HIST1H2BJ, H2BFR|
|Target/Specificity||This HIST1H2BJ/HIST1H2BK/HIST3H2BB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the C-terminal region of human HIST1H2BJ/HIST1H2BK/HIST3H2BB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIST1H2BJ/HIST1H2BK/HIST3H2BB Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.|
|Cellular Location||Nucleus. Chromosome.|
Author : Wang J1,Wang S1,Yan C1,Deng Y2,Huang Z2,Shi P1.
IUBMB Life. 2018 Aug 9. doi: 10.1002/iub.1873. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].
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