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>   首页   >   产品   >   一抗   >   细胞生物学   >   UBE2L3 Antibody (C-term)   

UBE2L3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - UBE2L3 Antibody (C-term) AP19885b
    Immunohistochemical analysis of paraffin-embedded H.kidney section using UBE2L3 Antibody (C-term)(Cat#AP19885b). AP19885b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - UBE2L3 Antibody (C-term) AP19885b
    UBE2L3 Antibody (C-term) (Cat. #AP19885b) western blot analysis in K562,U87-MG,mouse NIH/3T3,rat PC-12 cell line and mouse testis,rat testis tissue lysates (35ug/lane).This demonstrates the UBE2L3 antibody detected the UBE2L3 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P68036
Other Accession P68037, Q3MHP1, NP_003338.1
Reactivity Human, Rat, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 17862 Da
Antigen Region 106-135 aa
Additional Information
Gene ID 7332
Other Names Ubiquitin-conjugating enzyme E2 L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3, UBE2L3, UBCE7, UBCH7
Target/Specificity This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the C-terminal region of human UBE2L3.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE2L3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2L3
Synonyms UBCE7, UBCH7
Function Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine; in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates ubiquitination by the CUL9-RBX1 complex (PubMed:38605244). In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
Cellular Location Nucleus. Cytoplasm
Tissue Location Ubiquitous, with highest expression in testis.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.

REFERENCES

Fransen, K., et al. Hum. Mol. Genet. 19(17):3482-3488(2010)
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Purbeck, C., et al. Biochemistry 49(7):1361-1363(2010)
Han, J.W., et al. Nat. Genet. 41(11):1234-1237(2009)

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